CSM Single Drop out formulation-csm-single:CSM, Single Drop-Out -Iso: 740 mg/l 55 gram powdered medium in

55 gram powdered medium in 1 litre distilled water

1% Heavy metals >10ppm Residue on ignition >0

01 Folic Acid 0

Appearance White Crystalline Powder Identification (FTIR) Positive Solubility (100 mg/L) Clear and colourless solution Purity (HPLC) >92% Potency >1000 µg/mg Heavy metals <20 ppm Specific rotation 22

Friehs and Flaschel (2004)

CSM Single Drop out formulation-csm-single:CSM, Single Drop-Out -Iso: 740 mg/l 55 gram powdered medium inCSM is also a basis for many Drop Out mixtures to select for auxotrophic requirements and transformants. Each CSM Single Drop Out mixture contain all components except for one essential nutrient, i. e. the dropped out supplement. Some CSM Single Drop Out mixtures are also available with additions of extra Amino Acids and other components. Store all Drop out mixtures dry at room temperature