Sporulation Medium Size:250g

Sporulation Medium Size:250gBased on its constituents and the presence of Potassium acetate Sporulation medium is used to induce the induction of spores forming by yeast. Component g l Yeast extract 10 Glucose 0. 2 Potassium Acetate 10 Agar 20 Suspend 40. 2 gram powdered medium in 1 litre distilled water Store dry at room temperature.

coli strains are provided by LB Broth

1 g/l) Assay ≥99%

coli cells and lambda bacteriophages are obtained

K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium

Tryptone 10 Yeast extract 5 NaCl 5 MgSO4 anhydrous 0

5 Suspend 35

Carbenicillin is a beta-lactam type inhibitor of bacterial cell wall synthesis

Yeast extract 10 Glucose 100 Potassium Acetate 20 Agar 20 Suspend 150 gram powdered medium in 1 litre distilled water

0 Yeast Extract 2

2 at 25°C Suspend 25 gram powdered medium in 1 litre distilled water

2 at 25°C Suspend 15

2 Na2HPO4 0